One step一步法完成IP | Co-IP | ChIP,讓您的免疫沉淀更簡捷,更可靠,更專業
德國Chromotek 產品 —— 北京樂博生物中國區授權代理
GFP-Trap_A (產品貨號:gta-20)
產品描述: 與agarose beads結合
particle size ~ 80 µm
存儲緩沖液:20% EtOH
儲存方法:室溫運輸,收到后4℃儲存一年。請勿冰凍。
Nano-Traps®值得信賴的科研工具
Chromotek的Nano-Trap®基于alpaca的單域抗體片段(VHHS)
√ 小型
√ 極端穩定
√ 70℃仍保持穩定,functional in 2M NaCl or 0.5% SDS
√ 高親和力
√ 解離常數范圍在sub-nM內
√ 高特異性
GFP-Trap® 產品特點(高穩定性、高親和力)
特異性結合綠色熒光蛋白的衍生物,如GFPS65T,YPF或EYFP,不與DsRed衍生蛋白結合。(見FAQs)
√ 多功能且功能強大的GFP融合蛋白生化實驗
√ 縮短孵化時間(只需5-30min)
√ 從細胞提取物或細胞器中定量分離融合蛋白及結合(暫時性)因子
√ 超低的非特異性結合影響
√ 無污染的普通抗體的輕鏈與重鏈
√ 適用于染色質免疫沉淀(ChIP)
GFP-Trap® 產品應用:
√ 免疫沉淀 IP / Co-IP
√ 質譜分析
√ 酶活測定
√ 生物大分子相互作用/親和力實驗
√ 染色質免疫沉淀(ChIP,Chromatin Immunoprecipitation)
Nano-Traps reliable research reagents
NanoTraps are based on recombinant single domain antibody fragments (VHHS) derived from
alpaca coupled to monovalent matrices (agarose beads, magnetic particles or multiwell plates).
Our GFPTrap® / RFPTrap® are novel high quality tools for the fast, reliable and efficient onestep
isolation of fluorescent fusion proteins and their interacting factors.
Features
· Robust and versatile tools for biochemical analyses of fluorescent fusion proteins
· Short incubation times (5 – 30 min)
· Quantitative isolation of fusion proteins and transiently bound factors from various cell extracts
and organelles (from all organisms)
· Low unspecific binding
· No unspecific binding or contamination by heavy and light chains of conventional antibodies
GFP-Trap®
Our GFP-Trap® is a high quality GFP-binding protein coupled to a monovalent matrix (agarose beads,
magnetic particles or in 96-multiwell plates) for biochemical analysis of GFP fusion proteins and
their interacting partners.
The GFP-Trap® specifically binds to most common GFP derivates (see FAQs for a complete list).
No binding to red fluorescent proteins derived from DsRed can be detected.
GFP-Trap® features at a glance:
· Robust and versatile tool for biochemical analyses of GFP-fusion proteins
· Short incubation times (5 – 30 min)
· Quantitative isolation of fusion proteins and transiently bound factors from cell extracts or organelles
· Low unspecifi c binding
· No contaminating heavy and light chains of conventional antibodies
· Applicable in Chromatin Immunoprecipitation (ChIP)
Use GFP-Trap® for:
· Pulldowns/ Immunoprecipitations
· Co-IP
· Mass spectroscopy
· Enzyme activity measurements
· ChIP analysis
GFP-Trap_A
Description
GFP-Trap® coupled to agarose beads
particle size ~ 80 µm
Storage buffer
20% EtOH
Storage instructions
Shipped at ambient temperature. Upon receipt store at 4°C; stable for one
year. Do not freeze.
Latest references
- Castello, A. Fischer, B. Eichelbaum, K. Horos, R. Beckmann, B. Strein, C. Davey, N. Humphreys, D. Preiss, T. Steinmetz, L. Krijgsveld, J Hentze, M. (2012). Insights into RNA Biology from an Atlas of Mammalian mRNA-Binding Proteins. Cell (PubMed)
- Ries, J. Kaplan, C. Platonova, E. Eghidi, H. Ewers, H. (2012). A simple, versatile method for GFP-base super-resolution microscopy via nanobodies. Nat. Methods. 10.1038. (PubMed)
- Pichler, G. Jack, A. Wolf, P. Hake, S. B. (2012). Versatile Toolbox for High Throughput Biochemical and Functional Studies with Fluorescent Fusion Proteins. PLoS One. 7: e36967. (PubMed)
- Gudesblat, G. E. Schneider-Pizon, J. Betti, C. Mayerhofer, J. Vanhoutte, I. van Dongen, W. Boeren, S. Zhiponova, M. de Vries, S. Jonak, C. Russinova, E. (2012). SPEECHLESS integrates brassinosteroid and stomata signalling pathways. Nat Cell Biol. (PubMed)
- Majumdar, A. Cesario, W. C. White-Grindley, E. Jiang, H. Ren, F. Khan, M. R. Li, L. Choi, E. M. Kannan, K. Guo, F. Unruh, J. Slaughter, B. Si, K. (2012). Critical role of amyloid-like oligomers of Drosophila Orb2 in the persistence of memory. Cell. 148: 515-29. (PubMed)
- Metzger, T. Gache, V. Xu, M. Cadot, B. Folker, E. S. Richardson, B. E. Gomes, E. R. Baylies, M. K. (2012). MAP and kinesin-dependent nuclear positioning is required for skeletal muscle function. Nature. (PubMed)
- Wild, P. Farhan, H. McEwan, D. G. Wagner, S. Rogov, V. V. Brady, N. R. Richter, B. Korac, J. Waidmann, O. Choudhary, C. Dotsch, V. Bumann, D. Dikic, I. (2011). Phosphorylation of the autophagy receptor optineurin restricts Salmonella growth. Science. 333: 228-33. (PubMed)
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